Inhibition of DNA replication in vitro by pefloxacin

Effect of Host Src-kinase Inhibition on Replication of Coxsackievirus B3 in vitro

Inhibition of DNA replication by tirapazamine.

Tirapazamine (TPZ) is a hypoxia-selective cytotoxin that is currently being examined in Phase II and III clinical trials in combination with radiotherapy and cisplatin-based chemotherapy. Reductases convert TPZ to a cytotoxic radical that produces DNA damage under hypoxic conditions. Because one or more of the enzymes responsible for the bioactivation of TPZ is/are thought to be at or near the ...

In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi

Cyprinid herpesvirus-3 (CyHV-3) is an etiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp Cyprinus carpio L. There is no current treatment strategy to save CyHV-3 infected fish. RNA mediated interference (RNAi) is an emerging strategy used for understanding gene function and is a promising method in developing novel therapeutics and ...

Replication of damaged DNA in vitro is blocked by p53.

The tumor suppressor protein p53 may have other roles and functions in addition to its well-documented ability to serve as a sequence-specific transcriptional activator in response to DNA damage. We showed previously that p53 can block the replication of polyomavirus origin-containing DNA (Py ori-DNA) in vitro when p53 binding sites are present on the late side of the Py ori. Here we have both ...

Replication of Mammalian Dna in Vitro

We have used fiber autoradiography to examine the DNA product made in vitro in a lysed cell system . CHO cells were treated with 0.01% Brij-58 and the lysates were incubated at 30°C in a complete reaction mixture for in vitro DNA synthesis with [ 3H)thymidine triphosphate ([ 3H]TTP) as the radioactive tracer. Fiber autoradiograms prepared from the DNA showed that it was synthesized on tandemly ...